FIG 3.

Alginate synthesis is not required for S. aureus protection. S. aureus JE2 was cocultured in 1/2× MEM plus l-Gln and l-Arg on plastic with P. aeruginosa PAO1 or P. aeruginosa PAO1 algD::FRT with or without 1% seaweed-derived alginate. (A and B) S. aureus JE2 viability in the biofilm (A) and planktonic (B) fractions after 16 h. (C and D) P. aeruginosa PAO1 viability in the biofilm (C) and planktonic (D) fractions after 16 h. Significance was determined by one-way ANOVA with Dunnett’s posttest. a, P < 0.05 with S. aureus JE2 as the reference; b, P < 0.05 with P. aeruginosa PAO1 plus S. aureus JE2 as the reference; bd, below detection.