FIG 1.

Maps of pCF10 and the pCF10 prgX-prgQ regulatory region. Transcripts shown are expressed from promoter P_Q, with or without induction by C. (A) Map showing the extended Q_Op transcript and genes for which HCR transcript-labeling probes were designed. Early Q_Op transcripts (Q_L prgA, and prgB) for which HCR probes were designed are boxed in blue, and late Q_Op transcripts (prgJ, pcfC, and pcfG) for which HCR probes were designed are boxed in orange. (B) In the uninduced state, P_Q is modestly active and produces Q_S transcripts (red), which terminate at IRS1. Upon induction, production of Q_L (gray) and Q_Op transcripts (blue) increases dramatically. Q_L transcripts have are identical to Q_S transcripts through IRS1 but terminate at IRS2; for this study, we used HCR probes to RNAs produced from the sequences between IRS1 and IRS2, since they are unique to induced cells. Q_Op transcripts (blue) continue through regions of pCF10 that encode genes required for conjugation. prgX transcripts are generated from the opposite strand and extend from promoter P_x, terminating near the 3′ end of prgX (green lollipop). I, iCF10.