Skip to main content
. 2020 Mar 19;9(5):233–244. doi: 10.1089/wound.2018.0890

Figure 1.

Figure 1.

Verification of Sufu overexpression in the epidermis of TG mice. (a) Sufu expression vector. The Sufu cDNA was inserted into the K14 expression plasmid comprising the human K14 promoter and the K14 polyA sequence. Sufu overexpression in the epidermis of hSufu TG mice was confirmed by (b) polymerase chain reaction and (c) Western blot analyses. (d) Immunofluorescence staining revealed elevated Sufu levels in the skin of neonatal and 7-week-old mice localized in epidermal keratinocytes and hair follicle cells (Sufu—red, DAPI—blue). (e) Immunofluorescence staining revealed elevated Sufu levels in the tail skin of neonatal and 7-week-old mice localized in epidermal keratinocytes (Sufu—red, DAPI—blue). Scale bar = 0.1 mm. DAPI, 4′,6-diamidino-2-phenylindole; hSufu, human suppressor of fused; TG, transgenic. Color images are available online.