Skip to main content
. 2020 Mar 19;9(5):233–244. doi: 10.1089/wound.2018.0890

Figure 5.

Figure 5.

Sufu overexpression in the epidermis affects the dermis. (a) Sufu expression in the primary dermal fibroblasts was similar in NTG and NWT control mice and 7-week-old TG and WT mice. (b) Scratch wound experiments revealed no significant differences in migration distance between Sufu TG primary and control fibroblasts. (c) CCK8 analysis revealed increased proliferation capability in TG fibroblasts compared with control fibroblasts at 24 and 48 h. (d) Immunofluorescence revealed that the morphology of WT fibroblasts differentiated into contractile myofibroblasts in the presence of TGF-β1, whereas the morphology of TG fibroblasts did not change (SMA—red; DAPI—blue). TGF-β treatment increased the expression of α-SMA in WT fibroblasts compared with TG fibroblasts. GAPDH was used as a loading control. *p < 0.05. α-SMA, α-smooth muscle actin; CCK8, cholecystokinin octapeptide; DAPI, 4′,6-diamidino-2-phenylindole; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; NTG, neonatal TG; NWT, neonatal WT; Sufu, suppressor of fused; TG, transgenic; TGF-β1, transforming growth factor-β1; WT, wild type. Color images are available online.