Overexpression of Sufu retards wound healing via blocking the Hh/Gli and Wnt/β-catenin pathway. (a) Representative immunofluorescence staining images (n = 5 independent experiments) for β-catenin in control and TG wounds at day 7 and 12 postwounding. (b–d) Western blot analyses of β-catenin, MMP-3, and Gli1 at day 7 and 12 postinjury in the control and TG wounds. Gli, glioma transcription factor; Hh, hedgehog; MMP-3, matrix metalloproteinase-3; Sufu, suppressor of fused; TG, transgenic. Color images are available online.