Figure 3.

eBDPCs express Fgf9 protein in vitro. The eBDPCs synthesize and secrete Fgf9 protein. (A) The co-expression of CD34 (red fluorescent) and Fgf9 protein (green fluorescent) in eBDPCs in 2 weeks of coculture. (B) More than 96% of CD34⁺ cells express Fgf9 protein among the total eBDPCs. (C) ELISA shows a dramatic increase of Fgf9 protein level in media collected from eBDPC culture dish. The addition of fetal bovine serum did not influence the level of Fgf9 in the supernatant. (***p < 0.001) (D) Fgf9 protein mainly located in the cytoplasm and increases over time (red). Scale bar = 10 μm. ELISA, enzyme-linked immunosorbent assay.