Fig. 3.

Mice were exposed to 400 ppm Cl₂ (A and B) or 600 ppm Br₂ (C and D) for 30 min and returned to room air. At 1-h postexposure, the mice were injected with nifedipine (5 mg/kg body wt), diltiazem (5 mg/kg body wt), DMSO (100 µL), or saline subcutaneously. They were connected to a flexiVent FX at 24 h postexposure for measurements of airway resistance (R; prior to and after challenge with methacholine). A and C: values are means ± 1 SE. B and D: corresponding changes in airway resistance for Cl₂ and Br₂ exposed mice at a methacholine concentration of 40 mg/mL to enable statistical comparisons. Individual values and means ± 1 SE; number of mice in each group as follows: A and B: eight mice per group except for Cl₂+Diltiazem where n = 6; C and D: n = 8 for each group. Statistical analysis for the data shown in B and D was performed by one-way ANOVA followed by the Tukey test.