Fig. 10.

Top: uncropped version of Fig. 4D. Human airway smooth muscle cells (hASMC) were transfected with a scrambled siRNA (scr-siRNA; lanes 1, 2, 5, and 6) or a calcium-sensing receptor (Ca-SR) siRNA (lanes 3, 4, 7, and 8) and exposed to air (lanes 1–4) or Br₂ (100 ppm for 10 min; lanes 5–8) and returned to an incubator at 37°C in vented with 95% air and 5% CO₂ for 24 h. At that time, cells were processed for Western blotting analysis with a Ca-SR antibody as described in materials and methods. Entire gels are shown. There was a significant increase of Ca-SR protein in Br₂-exposed cells transfected with scrambled siRNA (lanes 5, 6), but not in those transfected with Ca-SR siRNA (lanes 7, 8). Bottom: uncropped version of Fig. 5G. Western blots of primary mouse airway cells (mpASMC) exposed to air or Br₂. Twenty-four hours postexposure, cells were processed for Western blotting with a primary antibody against the Ca-SR protein. Each blot was repeated twice.