Fig. 10.

Effects of red blood cell (RBC) supernatants (SUP) on LPS or TNF-α-induced inflammatory response of human pulmonary artery endothelial cells (HPAECs). A and B: HPAECs were treated with LPS (A) or TNF-α (B) for 1 h followed by addition of fresh RBC supernatants (1:100 dilution) for 16 to 20 h and ELISA analysis of the markers of inflammation: soluble ICAM-1 (sICAM-1), IL-8, and IL-6; n = 3. *P < 0.05 vs. LPS or TNF-α alone. C: Western blot analysis of ICAM-1 and VCAM-1 expression was performed in HPAEC challenged with LPS or TNF-α with or without posttreatment with fresh RBC supernatants. D: effects of fresh and old RBC supernatants on VCAM-1 and ICAM-1 expression caused by LPS or TNF-α. Membrane reprobing for β-tubulin was used as normalization control. Sf, fresh supernatant, less than 14 days; So, old supernatant, more than 28 days Bar graphs depict the quantitative densitometry analysis of Western blot data; n = 3. *P < 0.05.