Fig. 3.

Flow cytometry of immune cell populations in Achilles tendons after tenotomy and repair. A: dot plot demonstrating gating strategies. B: quantification of populations of monocytes (CD11b⁺/Ly6C^hi), neutrophils (CD11b⁺/Ly6C^med/SSC^hi), macrophages (CD11b⁺/Ly6C^lo/CD64⁺), dendritic cells (CD11c⁺/MHC II⁺), B cells (CD11b⁻/CD11c⁻/B220⁺), CD4⁺ T cells (CD3⁺/CD4⁺/CD8⁻), and CD8⁺ T cells (CD3⁺/CD4⁻/CD8⁺) from Achilles tendons. Mean values of sham-operated mice at 1wk (dark gray) or 2 wk (light gray) are shown as horizontal dotted lines but are not directly included in the statistical model. Differences between groups tested were obtained using a one-way ANOVA (α = 0.05) followed by Bonferroni post hoc sorting, as well as the Kruskal-Wallis test followed by Dunn’s multiple comparisons test. *Significantly different, P < 0.05, from control (Ctrl). †Significantly different, P < 0.05, from 1 wk. ‡Significantly different, P < 0.05, from 2 wk. n ≥ 3 mice per control or surgical time point.