Fig. 5.

Flow cytometry of immune cell populations in popliteal lymph nodes after Achilles tenotomy and repair. A: dot plot demonstrating gating strategies. B: quantification of populations of monocytes (CD11b⁺/Ly6C^hi), neutrophils (CD11b⁺/Ly6C^med/SSC^hi), macrophages (CD11b⁺/Ly6C^lo/CD64⁺), migratory dendritic cells (CD11c^med/MHC II^hi), resident dendritic cells (CD11c^hi/MHC II^med), plasma cells (CD11b⁻/CD11c⁻/B220⁻/IgG⁺), B cells (CD11b⁻/CD11c⁻/B220⁺/IgG⁻), CD4⁺ T cells (CD3⁺/CD4⁺/CD8⁻), and CD8⁺ T cells (CD3⁺/CD4⁻/CD8⁺) from popliteal lymph nodes. Mean values of sham operated mice at 1 wk (1w; dark gray) or 2 wk (2w; light gray) are shown as horizontal dotted lines but are not directly included in the statistical model. Differences between groups tested using a one-way ANOVA (α = 0.05) followed by Bonferroni post hoc sorting, as well as Kruskal-Wallis test followed by Dunn’s multiple comparisons: *Significantly different, P < 0.05, from control (Ctrl). †Significantly different, P < 0.05, from 1w. ‡Significantly different, P < 0.05, from 2w. n ≥ 3 mice per control or surgical time point.