Fig. 4.

Tandem pore domain acid-sensitive K⁺ channel 1 (TASK-1) global knockout (KO) mice have enhanced cardiac AKT phosphorylation. Immunoblot analysis results showing the phosphorylation of AKT in cardiac left ventricular samples obtained from wild-type, TASK-1 KO, and TASK-3 KO mice that have undergone a Sham procedure (Sh) or 3 days post-transverse aortic constriction (TAC) procedure (3D) and 7 days post-TAC procedure (7D). Average phosphorylation (phosphorylated protein/total protein) is expressed as fold change from wild-type Sham. Quantified average phosphorylation is shown at in panel at top, and representative Western blots are shown in panel at bottom. Error bars reflect means ± SE; n = no. of mice. Statistical comparisons were made using two-way repeated measures ANOVA. The P values for the interaction between genotype and days post-TAC are shown. Comparisons between genotypes at each time point were made using Bonferroni’s test for multiple comparisons, *P < 0.05 vs. wild type, †P < 0.05 vs. TASK-3 at each time point.