Table 1.
TASK-1 global knockout TAC experiment hemodynamics
| Wild Type |
TASK-1 KO |
|||
|---|---|---|---|---|
| Sham | TAC | Sham | TAC | |
| n | 6 | 8–9 | 5 | 12 |
| TAC gradient, mmHg | NA | 54 ± 6.8 | NA | 46 ± 6.0 |
| Heart rate, beats/min | 397 ± 35 | 362 ± 24 | 443 ± 19 | 415 ± 24 |
| LVESP, mmHg | 131 ± 18 | 164 ± 15 | 125 ± 11 | 171 ± 11 |
| LVEDP, mmHg | 7.3 ± 2.2 | 6.8 ± 0.8 | 5.4 ± 0.4 | 5.9 ± 0.7 |
| LVESV, μL | 15 ± 3.0 | 27 ± 4.5 | 18 ± 3.4 | 13 ± 3.2* |
| LVEDV, μL | 31 ± 3.9 | 40 ± 3.6 | 37 ± 3.9 | 34 ± 2.8 |
| Arterial elastance, mmHg/μL | 8.6 ± 1.8 | 14.0 ± 2.5 | 6.6 ± 0.8 | 8.0 ± 0.93* |
| Systolic function parameters | ||||
| Stroke volume, μL | 16 ± 1.9 | 13 ± 1.6 | 19 ± 1.9 | 20.0 ± 1.6* |
| Ejection fraction, % | 55 ± 7.9 | 36 ± 7.5 | 52 ± 6.1 | 65.0 ± 6.6* |
| Cardiac output, μL/min | 6,334 ± 894 | 4,588 ± 561 | 8,232 ± 722 | 8,331 ± 587* |
| Stroke work, mmHg·μL | 1,555 ± 306 | 1,427 ± 254 | 1,672 ± 214 | 2,621 ± 268* |
| dP/dtmax, mmHg/s | 10,142 ± 1,978 | 6,718 ± 735 | 9,359 ± 570 | 9,289 ± 592 |
| ESPVR linear, mmHg/μL | 5.0 ± 0.8 | 6.1 ± 1.0 | 5.2 ± 1.4 | 5.9 ± 0.6 |
| ESPVR quadratic E′max, mmHg/μL | 14.0 ± 4.6 | 12.0 ± 1.9 | 13.0 ± 2.0 | 16.0 ± 1.3 |
| PRSW, mmHg | 52.0 ± 6.2 | 67.0 ± 8.5 | 54.0 ± 7.2 | 83.0 ± 6.3 |
| dP/dtmax vs. EDV, mmHg·s−1·μL−1 | 280 ± 120 | 201 ± 33 | 284 ± 85 | 225 ± 36 |
| Emax, mmHg/μL | 11.0 ± 3.7 | 9.2 ± 1.5 | 11.0 ± 3.4 | 9.7 ± 0.8 |
| Diastolic function parameters | ||||
| dP/dtmin, mmHg/s | 8,101 ± 1,645 | −6,137 ± 713 | −7,547 ± 900 | −8,479 ± 521 |
| τ (Weiss), ms | 9.1 ± 1.0 | 11.0 ± 0.9 | 8.6 ± 0.6 | 7.3 ± 0.4* |
| τ (Glantz), ms | 15.0 ± 2.5 | 20.0 ± 2.5 | 13.0 ± 0.5 | 12.0 ± 0.5* |
| τ (log), ms | 7.4 ± 0.8 | 9.2 ± 0.9 | 6.8 ± 0.2 | 6.0 ± 0.3* |
| dV/dtmin, μL/s | −431 ± 45 | −349 ± 46 | −564 ± 49 | −576 ± 64* |
| EDPVR linear, mmHg/μL | 0.30 ± 0.06 | 0.39 ± 0.10 | 0.23 ± 0.06 | 0.27 ± 0.04 |
| β-Coefficient | 0.11 ± 0.04 | 0.13 ± 0.03 | 0.06 ± 0.01 | 0.08 ± 0.01 |
Values are means ± SE; n = no. of mice. Average load-dependent and load-independent hemodynamic parameters obtain invasive pressure-volume loop analyses in wild-type and tandem pore domain acid-sensitive K+ channel 1 (TASK-1) global knockout (KO) mice that underwent either a sham procedure (Sham) or pressure overload using transverse aortic constriction (TAC). dP/dtmax and dP/dtmin, maximum and minimum dP/dt, respectively; dV/dtmin, minimum dV/dt; EDPVR, end-diastolic pressure-volume relationship; EDV, end-diastolic volume; Emax, maximal elastance; E′max, maximum slope of quadratic end-systolic pressure-volume relationship (ESPVR); LVEDP, left ventricular end-diastolic pressure; LVEDV, left ventricular end-diastolic volume; LVESP, left ventricular end-systolic pressure; LVESV, left ventricular end-systolic volume; NA, not applicable; PRSW, preload recruitable stroke work; τ, time constant of left ventricular isovolumic relaxation. Statistical comparisons between TAC gradients were made using a 2-tailed unpaired t-test, and all others were made using a 1-way ANOVA with Bonferroni’s test for multiple comparisons.
P < 0.05 vs. wild-type TAC.