Table 2.
TASK-3 global knockout TAC experiment hemodynamics
| Wild Type |
TASK-3 KO |
|||
|---|---|---|---|---|
| Sham | TAC | Sham | TAC | |
| n | 7 | 8 | 7 | 13–15 |
| TAC gradient, mmHg | NA | NA | 67 ± 12.0 | 77 ± 8.7 |
| Heart rate, beats/min | 503 ± 35 | 397 ± 15* | 520 ± 36 | 402 ± 15* |
| LVESP, mmHg | 105 ± 9 | 188 ± 14* | 103 ± 13 | 172 ± 12* |
| LVEDP, mmHg | 13.0 ± 2.8 | 5.3 ± 1.9 | 11.0 ± 1.3 | 13.0 ± 1.5 |
| LVESV, μL | 11.0 ± 2.8 | 26.0 ± 6.1 | 33.0 ± 5.3 | 35.0 ± 5.4 |
| LVEDV, μL | 36.0 ± 3.4 | 46.0 ± 7.2 | 54.0 ± 6.8 | 55.0 ± 5.3 |
| Arterial elastance, mmHg/μL | 3.9 ± 0.5 | 13.0 ± 4.0 | 5.2 ± 0.6 | 11.0 ± 1.9 |
| Systolic function parameters | ||||
| Stroke volume, μL | 25 ± 1.6 | 19 ± 3.1 | 21 ± 3.1 | 19 ± 2.2 |
| Ejection fraction, % | 72 ± 5.2 | 46 ± 7.4* | 40 ± 3.9* | 39 ± 5.3 |
| Cardiac output, μL/min | 2,928 ± 1,487 | 7399 ± 1,111* | 10,440 ± 1,171 | 7,407 ± 670 |
| Stroke work, mmHg·μL | 1,992 ± 122 | 2,550 ± 488 | 1,712 ± 438 | 2,133 ± 206 |
| dP/dtmax, mmHg/s | 8,309 ± 859 | 9,032 ± 714 | 7,573 ± 1,079 | 7,771 ± 472 |
| ESPVR linear, mmHg/μL | 4.2 ± 0.8 | 5.5 ± 1.2 | 5.3 ± 2.1 | 7.2 ± 1.8 |
| ESPVR quadratic E′max, mmHg/μL | 11.0 ± 1.5 | 13.0 ± 2.4 | 15.0 ± 4.1 | 14.0 ± 3.5 |
| PRSW, mmHg | 61.0 ± 5.5 | 59.0 ± 8.9 | 51.0 ± 6.9 | 54.0 ± 6.2 |
| dP/dtmax vs. EDV, mmHg·s−1·μL−1 | 177 ± 42 | 187 ± 45 | 194 ± 48 | 145 ± 20 |
| Emax, mmHg/μL | 7.5 ± 0.9 | 8.9 ± 2.0 | 9.9 ± 2.6 | 10.0 ± 2.5 |
| Diastolic function parameters | ||||
| dP/dtmin, mmHg/s | −8,580 ± 475 | −9,677 ± 737 | −7,162 ± 915 | −7,485 ± 525 |
| τ (Weiss), ms | 6.4 ± 0.6 | 7.9 ± 0.5 | 8.0 ± 0.6 | 8.9 ± 0.4 |
| τ (Glantz), ms | 11.0 ± 1.0 | 14.0 ± 1.1 | 14.0 ± 2.1 | 16.0 ± 1.2 |
| τ (log), ms | 5.3 ± 0.5 | 6.8 ± 0.5 | 5.9 ± 0.7 | 7.3 ± 0.4 |
| dV/dtmin, μL/s | −764 ± 59 | −493 ± 72 | −713 ± 86 | −649 ± 78 |
| EDPVR linear, mmHg/μL | 0.18 ± 0.04 | 0.43 ± 0.06* | 0.38 ± 0.04 | 0.46 ± 0.06 |
| β-Coefficient | 0.04 ± 0.01 | 0.07 ± 0.01 | 0.09 ± 0.02 | 0.07 ± 0.01 |
Values are means ± SE; n = no. of mice. Average load-dependent and load-independent hemodynamic parameters obtain invasive pressure-volume loop analyses in wild-type and tandem pore domain acid-sensitive K+ channel 3 (TASK-3) global knockout (KO) mice that underwent either a sham procedure (Sham) or pressure overload using transverse aortic constriction (TAC). dP/dtmax and dP/dtmin, maximum and minimum dP/dtmin, respectively; dV/dtmin, minimum dV/dt; EDPVR, end-diastolic pressure-volume relationship; EDV, end-diastolic volume; Emax, maximal elastance; E′max, maximum slope of quadratic end-systolic pressure-volume relationship; ESPVR, end-systolic pressure-volume relationship; LVEDP, left ventricular end-diastolic pressure; LVEDV, left ventricular end-diastolic volume; LVESP, left ventricular end-systolic pressure; LVESV, left ventricular end-systolic volume; PRSW, preload recruitable stroke work; τ, time constant of left ventricular isovolumic relaxation. Statistical comparisons between TAC gradients were made using a 2-tailed unpaired t-test, and all others were made using a 1-way ANOVA with Bonferroni’s test for multiple comparisons.
P < 0.05 vs. wild-type TAC.