Blockade of the arcuate nucleus with the insulin receptor antagonist S961 decreases lumbar sympathetic nerve activity (LSNA), heart rate (HR), and mean arterial pressure (MAP) in obesity-prone (OP, n = 5), but not obesity-resistant (OR, n = 5) and control (CON, n = 5), rats. Top: representative LSNA traces. Bottom: grouped data showing time course of changes (left) and maximal changes (right) in LSNA (at 67.5 ± 4.0 min), HR (at 72.7 ± 1.3 min), and MAP (at 66.3 ± 5.1 min). Two-way repeated-measures ANOVA revealed significant group (P = 0.005), time (P < 0.0001), and interaction (P < 0.0001) for LSNA; significant group (P < 0.005) and interaction (P < 0.0001), but not time, for HR; and significant group (P < 0.05), time (P < 0.01), and interaction (P = 0.0002) for MAP. *P < 0.05 vs. baseline (0); †P < 0.05 vs. OR and CON.