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Top: summary of paraventricular nucleus (PVN), arcuate nucleus (ArcN), and dorsal medial hypothalamus (DMH) nanoinjection sites. ●, Control (CON); □, obesity-resistant (OR); solid gray triangles, obesity-prone (OP). MTII, melanotan II. [Histological maps were hand drawn with the Paxinos and Watson atlas (54) used as a guide.] Bottom: representative injection sites in the PVN (left), ArcN (middle), and DMH (right), visualized with fluorescent microbeads.
