Fig. 5.

The effect of macrophage depletion on liver repair of fibrotic liver after I/R. A: mice with fibrotic liver underwent ischemia-reperfusion (I/R) and got injected with clodronate liposome at 24 h and 72 h after surgery. B: macrophages in the liver were identified by staining liver sections with F4/80. Clodronate liposome injection decreased the accumulation of macrophages at 96 h after I/R. Original magnification is ×100. C: liver necrosis and parenchymal area were assessed by hematoxylin-and-eosin staining, and necrosis area was calculated. Clodronate liposome injection treatment delayed the clearance of necrotic tissue and liver regeneration after I/R. Original magnification is ×100. Data are expressed as means ± SE with n = 4–5 mice per group. *P < 0.05 compared with control.