Fig. 4.

High-fructose, high-fat (HFF)-fed pigs had decreased phosphatidylcholines (PCs) and increased phosphatidylethanolamines (PEs), lysophosphatidylethanolamines (LPEs), and lysophosphatidylcholines (LPCs) in liver. Heat map is shown of absolute abundance of lipids significantly altered by the diet in plasma (A) and liver (B), along with individual fatty acids in liver (C) of 83-day-old pigs, with fold change and significance levels by HFF compared with control (CON). Lipidome and fatty acids analyses were performed by mass spectrometry and gas chromatography, respectively. Columns are individual pigs, and rows are log₂-transformed metabolites. Blue and red represent the row minimum and maximum values, respectively. P values for each metabolite were calculated by a two-way ANOVA with a mixed model and adjusted for multiple testing with Benjamin-Hochberg procedure. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.