Fig. 1.

Kidney morphology in mice after ischemia-reperfusion injury (IRI). Wild-type mice were subjected to sham (A and C) or 45-min ischemia (B and D) operations. Two days after surgery, mice were euthanized, and kidneys were harvested for the evaluation of kidney morphological changes with hematoxylin and eosin (HE) staining (A and B) and periodic acid-Schiff (PAS) staining (C and D). Scale bar = 100 μm. The closed arrow depicts normal or nearly normal renal tubules; the open arrow depicts necrotic changes in renal tubules, including loss of cell nuclei, detached cell debris, and protein casts.