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. 2020 Jan 27;318(3):F772–F792. doi: 10.1152/ajprenal.00504.2019

Fig. 7.

Fig. 7.

Coimmunoprecipitation of the beclin 1/Bcl-2 complex in the kidney with anti-Bcl-2 or anti-beclin 1 antibodies. A: total kidney lysates from two normal wild-type (WT) mice were immunoprecipitated with mouse anti-Bcl-2 antibody or normal mouse IgG or lysate buffer and then immunoblotted with beclin 1 and Bcl-2 antibodies, respectively. Immunoblots (IBs) for beclin 1 and Bcl-2 protein in input proteins were shown. B: total kidney lysates from two normal WT mice were immunoprecipitated with mouse anti-beclin 1 antibody or normal mouse IgG or lysate buffer and then immunoblotted with beclin 1 and Bcl-2 antibodies, respectively. IBs for beclin 1 and Bcl-2 protein in input proteins were shown. Three independent experiments were repeated.