Table 4.
Phosphopeptides that showed abundance changes in opposite directions in response to deletion of MLCK versus PKA
| Gene Symbol | Site(s) | Centralized Sequence | Log2(MLCK null/MLCK intact) | Log2(PKA null/PKA intact) |
|---|---|---|---|---|
| Ranbp2 | S954† | PATGILS*PRGDDY | −1.43 | 0.71 |
| Cap1 | S34† | HCGYGDS*PSKGAV | −1.03 | 3.73 |
| Kpna2 | S62† | FPDDATS*PLQENR | −0.95 | 0.81 |
| Smtn12 | S339 | KLKRSQS*FGVASA | −0.68 | 0.49 |
| Cep55 | S40 | IAAFKTS*LDEITS | −0.65 | 1.51 |
| Scaf1 | S518† | DPAPPDS*PSWEAK | −0.61 | 0.42 |
| Tax1bp1 | S632† | RKLEGQS*PQQVSR | −0.54 | 0.62 |
| Cep170b | S709† | PSGRADS*PAGLEA | −0.47 | 1.23 |
| Rtn4 | S857† | VSAKDDS*PKEYTD | −0.39 | 0.67 |
| Eif4g1 | S1081 | KITKPGS*IDSNNQ | −0.39 | 0.29 |
| Mdc1 | S168 | VLLAADS*EEEGDF | −0.38 | 0.74 |
| Myh10 | S1956 | GASLELS*DDDTES | −0.37 | 0.54 |
| Sptbn1 | S2340† | TITSESS*PGKREK | −0.34 | 1.04 |
| Kiaa1522 | S838, S842† | RRALS*GRAS*PVTA | −0.29 | 0.69 |
| Pgam1 | S118 | VKIWRRS*YDVPPP | −0.24 | 0.36 |
†
Sites with the motif X-p(S/T)-P. MLCK, myosin light chain kinase; Ranbp2, RAN-binding protein 2; Cap1, cyclase-associated actin cytoskeleton regulatory protein 1; Kpna2, karyopherin subunit α2; Smtn12, smoothelin-like protein 2; Cep55, centrosomal protein 55; Scaf1, splicing factor, arginine/serine-rich 19; Tax1bp1, Tax1-binding protein 1; Cep170b, centrosomal protein 170B; Rtn4, reticulon 4; Eif4g1, eukaryotic translation initiation factor 4γ; Mdc1, mediator of DNA damage checkpoint 1; Myh10, myosin heavy chain 10; Sptbn1, spectrin β-chain, brain 1; Pgam1, phosphoglycerate mutase 1.
*
Phosphorylated amino acid.