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. 2020 Jan 8;318(3):C675–C694. doi: 10.1152/ajpcell.00492.2019

Fig. 2.

Fig. 2.

Developmental principal cell mesh knockdown in the Drosophila Malpighian tubule using mesh RNA interference (RNAi) line 12074-R1 results in morphological defects in adult tubules. A and B: bright-field images with depiction of the major segments of the anterior Malpighian tubules from a 1-day-old adult control female fly (w;c42-GAL4/+; A) and developmental principal cell mesh knockdown fly (w;UAS-meshRNAi/+;c42-GAL4/+; B). The mesh knockdown tubules appear dilated compared with control tubules and accumulate opaque concretions in the initial segments. C–H: Mesh immunolocalizes to the smooth septate junctions (sSJs) between the epithelial cells throughout all segments in control tubules (C–E), but little to no Mesh immunoreactivity is observed in the initial/transitional, main, and lower segments and upper ureter of mesh knockdown tubules (F-H). Nuclei were counterstained blue with DAPI in C–H. Ten samples per genotype were examined. Scale bars, 100 µm (A and B) and 50 μm (C–H). The mesh RNAi line 12074-R1 was used in subsequent figures unless otherwise noted.