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. 2020 Jan 8;318(3):C675–C694. doi: 10.1152/ajpcell.00492.2019

Fig. 6.

Fig. 6.

Developmental principal cell mesh knockdown in the Drosophila Malpighian tubule does not alter the localization of Na+/K+-ATPase (NKA) or the organization of the actin cytoskeleton. The main segment of 1-day-old adult control female fly anterior tubules (w;c42-GAL4/+; A–A′′′ and C–C′′′) shows high expression of Mesh at bicellular contacts and NKA at the principal cells’ basolateral membrane and F-actin bundles that lie perpendicularly to the tubule long axis. A′′′ and C′′′ show single-confocal plane images indicated by dashed-box areas in A′′ and C′′, respectively. In principal cell mesh knockdown tubule main segments (w;UAS-meshRNAi/+;c42-GAL4/+; B–B′′′ and D–D′′′), NKA expression appears reduced but remains basolateral, and the expression and distribution of F-actin appear largely intact. B′′′ and D′′′ show single-confocal plane images indicated by dashed-box areas in B′′ and D′′, respectively. Nuclei were counterstained blue with DAPI. PC, principal cell; RNAi, RNA interference; SC, stellate cell. Ten samples per genotype were examined. Scale bars, 50 μm.