Table 1.
Secretion rate, [K+], potassium flux, and abdominal volume in developmental principal cell mesh knockdown using mesh RNAi line 12074-R1
| Genotype | Secretion Rate, nL/min | [K+], mM | K+ Flux, pmol·min−1·tubule−1 | n | Slope | Abdominal Volume, µL |
|---|---|---|---|---|---|---|
| Developmental principal cell mesh knockdown | ||||||
| GAL4 control | 0.61 ± 0.05 | 145 ± 8.2 | 92 ± 10.7 | 23 | 53.5 | 1.22 ± 0.02 |
| UAS control | 0.56 ± 0.05 | 148 ± 7.0 | 84 ± 9.2 | 22 | 1.29 ± 0.02 | |
| mesh Knockdown | 0 | 0 | 0 | 25 | 1.83 ± 0.04 | |
| One-way ANOVA P value | <0.0001 | <0.0001 | <0.0001 | <0.0001 | ||
| Tukey’s multiple comparison adjusted P values | ||||||
| GAL4 control vs. UAS control | 0.7828 | 0.9697 | 0.7801 | 0.2057 | ||
| GAL4 control vs. mesh knockdown | <0.0001 | <0.0001 | <0.0001 | <0.0001 | ||
| UAS control vs. mesh knockdown | <0.0001 | <0.0001 | <0.0001 | <0.0001 | ||
| Developmental principal cell mesh knockdown and drosokinin | ||||||
| GAL4 control, −DK | 1.16 ± 0.12 | 146 ± 3.5 | 170 ± 17.5 | 34 | 53.2 | |
| GAL4 control, +DK | 2.32 ± 0.09 | 154 ± 2.5 | 359 ± 14.4 | 34 | ||
| UAS control, −DK | 1.22 ± 0.08 | 146 ± 3.6 | 176 ± 12.2 | 34 | ||
| UAS control, +DK | 2.10 ± 0.09 | 146 ± 3.6 | 308 ± 15.3 | 33 | ||
| mesh Knockdown, −DK | 0.13 ± 0.05 | 79 ± 11.0 | 12 ± 5.3 | 5/35 | ||
| mesh Knockdown, +DK | 0.20 ± 0.05 | 118 ± 13.0 | 26 ± 6.9 | 7/35 | ||
| Two-way ANOVA P values | ||||||
| Genotype | <0.0001 | <0.0001 | <0.0001 | |||
| Drosokinin | <0.0001 | 0.0007 | <0.0001 | |||
| Interaction | 0.0055 | 0.0109 | 0.0028 | |||
| Sidak’s multiple comparison adjusted P values | ||||||
| GAL4 control, −DK vs. +DK | <0.0001 | 0.3021 | <0.0001 | |||
| UAS control, −DK vs. +DK | <0.0001 | 0.9997 | <0.0001 | |||
| mesh Knockdown, −DK vs. +DK | 0.9938 | 0.0033 | 0.9901 | |||
Values are means ± SE; n is the number of tubules analyzed. Genotypes are as follows (developmental principal cell mesh knockdown): GAL4 control, w;c42-GAL4/+; upstream activation sequence (UAS) control, w;UAS-meshRNAi/+; and mesh knockdown, w;UAS-meshRNAi/+;c42-GAL4/+. For mesh knockdown with and without drosokinin, the number of tubules secreting fluid and the total number of tubules analyzed are shown. “Slope” is the mean slope per 10-fold difference in [K+] of the electrodes used for each experiment. For abdominal volume, 21 flies per genotype were analyzed. DK, drosokinin; RNAi, RNA interference.