Table 2.
Secretion rate, [K+], and potassium flux in developmental principal cell mesh knockdown using mesh RNAi line 6867
| Genotype | Secretion Rate, nL/min | [K+], mM | K+ Flux, pmol·min−1·tubule−1 | n | Slope |
|---|---|---|---|---|---|
| Developmental principal cell mesh knockdown | |||||
| GAL4 control | 0.84 ± 0.06 | 135 ± 4.4 | 115 ± 9.5 | 17 | 51.9 |
| UAS control | 0.90 ± 0.04 | 134 ± 5.4 | 121 ± 7.8 | 17 | |
| mesh Knockdown | 0.35 ± 0.15 | 96 ± 14.9 | 45 ± 21.9 | 8/17 | |
| One-way ANOVA P value | <0.0001 | 0.0021 | 0.0003 | ||
| Tukey’s multiple comparison adjusted P values | |||||
| GAL4 control vs. UAS control | 0.8083 | 0.9906 | 0.9127 | ||
| GAL4 control vs. mesh knockdown | 0.0002 | 0.0029 | 0.001 | ||
| UAS control vs. mesh knockdown | <0.0001 | 0.0039 | 0.0004 | ||
| Developmental principal cell mesh knockdown and drosokinin | |||||
| GAL4 control, −DK | 0.75 ± 0.08 | 142 ± 7.8 | 102 ± 11.8 | 20 | 53.9 |
| GAL4 control, +DK | 1.93 ± 0.13 | 150 ± 4.5 | 288 ± 19.8 | 24 | |
| UAS control, −DK | 0.77 ± 0.09 | 147 ± 5.0 | 112 ± 14.9 | 23 | |
| UAS control, +DK | 1.44 ± 0.11 | 147 ± 6.1 | 212 ± 18.3 | 22 | |
| mesh Knockdown, −DK | 0.47 ± 0.11 | 132 ± 12.9 | 65 ± 17.6 | 7/24 | |
| mesh Knockdown, +DK | 0.42 ± 0.10 | 127 ± 13.5 | 57 ± 17.3 | 12/24 | |
| Two-way ANOVA P values | |||||
| Genotype | <0.0001 | 0.1097 | <0.0001 | ||
| Drosokinin | <0.0001 | 0.8378 | <0.0001 | ||
| Interaction | <0.0001 | 0.6918 | <0.0001 | ||
| Sidak’s multiple comparison adjusted P values | |||||
| GAL4 control, −DK vs. +DK | <0.0001 | <0.0001 | |||
| UAS control, −DK vs. +DK | <0.0001 | <0.0001 | |||
| mesh Knockdown, −DK vs. +DK | 0.9925 | 0.9945 | |||
| Contingency analysis | |||||
| Fisher’s exact test P value | 0.2375 |
Values are means ± SE; n is the number of tubules analyzed. See Table 1 legend for genotypes. For mesh knockdown with and without drosokinin, the number of tubules secreting fluid and the total number of tubules analyzed are shown. Fisher’s exact test indicates the P value for the difference between the number of secreting mesh knockdown tubules with and without drosokinin. “Slope” is the mean slope per 10-fold difference in [K+] of the electrodes used for each experiment. DK, drosokinin; RNAi, RNA interference; UAS, upstream activation sequence.