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. 2020 Jan 8;318(3):C675–C694. doi: 10.1152/ajpcell.00492.2019

Table 3.

Secretion rate, [K+], and potassium flux in mesh knockdown in adulthood

Genotype Secretion Rate, nL/min [K+], mM K+ Flux, pmol·min−1·tubule−1 n Slope
mesh Knockdown in adult principal cells
    GAL4 control 0.83 ± 0.04 146 ± 3.3 123 ± 7.1 35 51.8
    UAS control 0.80 ± 0.07 145 ± 3.0 117 ± 10.3 34
    mesh Knockdown 0.51 ± 0.04 133 ± 4.6 70 ± 6.6 34
One-way ANOVA P value <0.0001 0.0289 <0.0001
Tukey’s multiple comparison adjusted P values
    GAL4 control vs. UAS control 0.8605 0.9888 0.8462
    GAL4 control vs. mesh knockdown <0.0001 0.0446 <0.0001
    UAS control vs. mesh knockdown 0.0003 0.0654 0.0003
mesh Knockdown in adult principal cells and drosokinin
    GAL4 control, −DK 1.01 ± 0.07 148 ± 3.4 150 ± 11.8 36 54.7
    GAL4 control, +DK 2.33 ± 0.14 152 ± 2.5 352 ± 20.2 34
    UAS control, −DK 1.03 ± 0.09 153 ± 3.2 157 ± 14.9 34
    UAS control, +DK 2.87 ± 0.21 149 ± 3.7 429 ± 32.7 34
    mesh Knockdown, −DK 0.63 ± 0.07 138 ± 5.4 89 ± 10.7 38
    mesh Knockdown, +DK
Two-way ANOVA P values		 1.34 ± 0.11 138 ± 4.1 188 ± 16.7 33
    Genotype <0.0001 0.0016 <0.0001
    Drosokinin <0.0001 0.9623 <0.0001
    Interaction <0.0001 0.5389 <0.0001
Sidak’s multiple comparison adjusted P values
    GAL4 control, −DK vs. +DK <0.0001 0.8193 <0.0001
    UAS control, −DK vs. +DK <0.0001 0.8151 <0.0001
    mesh Knockdown, −DK vs. +DK 0.0001 0.9999 0.0008

Values are means ± SE; n is the number of tubules analyzed. Genotypes are as follows (mesh knockdown in adult principal cells): GAL4 control, w;tub-GAL80ts20/+;c42-GAL4/+; upstream activation sequence (UAS) control, w;UAS-meshRNAi/+; and mesh knockdown, w;tub-GAL80ts20/UAS-meshRNAi;c42-GAL4/+. The meshRNAi line used is 12074-R1. “Slope” is the mean slope per 10-fold difference in [K+] of the electrodes used for each experiment. DK, drosokinin.