Table 4.
Transepithelial and principal cell basolateral and apical membrane voltages and Cl− diffusion potential
| Genotype | Transepithelial Voltage, mV | Principal Cell Basolateral Membrane Voltage, mV | Principal Cell Apical Membrane Voltage, mV | Transepithelial Cl− Diffusion Potential, mV |
|---|---|---|---|---|
| mesh Knockdown in adult principal cells, −DK | ||||
| GAL4 control | 8.9 ± 4.2 (9) | −47.8 ± 8.2 (6) | 54.7 ± 7.0 (4) | 8.8 ± 7.8 (10) |
| mesh Knockdown | 5.2 ± 3.6 (20) | −49.9 ± 4.3 (9) | 55.1 ± 5.3 (8) | 7.9 ± 8.0 (15) |
| mesh Knockdown in adult principal cells, +DK | ||||
| GAL4 control | 3.2 ± 3.5 (9) | −49.3 ± 5.5 (6) | 50.8 ± 5.9 (4) | 17.0 ± 9.3 (10) |
| mesh Knockdown | 1.6 ± 3.4 (20) | −47.5 ± 1.8 (9) | 49.3 ± 5.8 (8) | 11.8 ± 10.4 (15) |
| Two-way ANOVA P values | ||||
| Genotype | 0.0204 | 0.7372 | 0.8915 | 0.3747 |
| Drosokinin | <0.0001 | 0.5260 | 0.0635 | 0.0005 |
| Interaction | 0.2686 | 0.1613 | ||
| Sidak’s multiple comparison adjusted P values | ||||
| −DK | ||||
| GAL4 control vs. mesh knockdown | 0.0231 | 0.9655 | ||
| +DK | ||||
| GAL4 control vs. mesh knockdown | 0.4402 | 0.2998 |
Values are means ± SD; number of tubules n is in parentheses. See Table 3 legend for genotypes. The meshRNAi line used is 12074-R1. Paired electrophysiological measurements (with and without 1 µM drosokinin) were made of control and adult principal cell mesh knockdown tubules. The apical membrane voltage was calculated as transepithelial voltage minus basolateral membrane voltage in tubules in which both values were measured. P values for repeated measures 2-way ANOVA are shown. DK, drosokinin.