Table 5.
Secretion rate, [FITC-dextran], and FITC-dextran flux
| Genotype | Secretion Rate, nL/min | [FITC-Dextran], ng/nL | FITC-Dextran Flux, pmol·min−1·tubule−1 | n |
|---|---|---|---|---|
| mesh Knockdown in adult principal cells and drosokinin | ||||
| GAL4 control, −DK | 1.10 ± 0.11 | 0.36 ± 0.03 | 0.071 ± 0.007 | 32 |
| GAL4 control, +DK | 1.92 ± 0.11 | 0.19 ± 0.01 | 0.072 ± 0.006 | 31 |
| UAS control, −DK | 1.20 ± 0.09 | 0.37 ± 0.03 | 0.077 ± 0.006 | 34 |
| UAS control, +DK | 1.99 ± 0.11 | 0.21 ± 0.02 | 0.081 ± 0.005 | 33 |
| mesh Knockdown, −DK | 0.57 ± 0.06 | 0.44 ± 0.04 | 0.043 ± 0.003 | 33 |
| mesh Knockdown, +DK | 1.04 ± 0.09 | 0.29 ± 0.03 | 0.049 ± 0.004 | 33 |
| Two-way ANOVA P values | ||||
| Genotype | <0.0001 | 0.0115 | <0.0001 | |
| Drosokinin | <0.0001 | <0.0001 | 0.4122 | |
| Interaction | 0.1212 | 0.9556 | 0.8692 | |
| Sidak’s multiple comparison adjusted P values | ||||
| −DK | ||||
| GAL4 control vs. UAS control | 0.7500 | 0.9947 | 0.8192 | |
| GAL4 control vs. mesh knockdown | 0.0004 | 0.1643 | 0.001 | |
| UAS control vs. mesh knockdown | <0.0001 | 0.1882 | <0.0001 | |
| +DK | ||||
| GAL4 control vs. UAS control | 0.8742 | 0.8693 | 0.5330 | |
| GAL4 control vs. mesh knockdown | <0.0001 | 0.0891 | 0.0128 | |
| UAS control vs. mesh knockdown | <0.0001 | 0.2320 | 0.0001 |
Values are means ± SE; n is number of tubules analyzed. See Table 3 legend for genotypes. The meshRNAi line used is 12074-R1. DK, drosokinin; UAS, upstream activation sequence.