Fig. 3.
Pretreatment with FPS-ZM1 (1 μM) attenuates high-mobility group box-1 protein (HMGB-1)-mediated increase in epithelial Na+ channel (ENaC) activity. A: small airway epithelial cells (SAECs) were pretreated with FPS-ZM1, an inhibitor of receptor for advanced glycation end products, 1 h before HMGB-1 (0.5 μg/mL) application to the extracellular bath. Inset: single-channel detail at −100 mV (–Vp) holding potential. B: calculated chord conductance (γ) and frequency histogram from representative trace in A. HSC, highly selective cation channel; NSC, nonselective cation channel. C: HMGB-1 fails to increase ENaC Po in SAECs pretreated with FPS-ZM1. Significant channel rundown was observed in both CF (n = 4) and normal (n = 4) SAECs. *P < 0.05, before vs. after HMGB-1 application in FPS-ZM1-pretreated SAECs (by paired t test).