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Forward and reverse primers used for real time RT-PCR.
| Genes | Forward primers (5′3′) | Reverse primers (5′–3′) |
|---|---|---|
| GAPDH | ACGACAGTCCATGCCATCAC | TCCACCCTGTTGCTGTA |
| Collagen 1 (COL1) | ACTGTCCCAACCCCCAAAG- | CGTATTCTTCCGGGCAGAAA |
| Osteocalcin (OCN) | CGGCCCTGAGTCTGACAAA | GCCGGAGTCTGTTCACTACCTT |
| Bone sialoprotein (BSP) | AACAATCCGTGCCACTCA | GGAGGGGGCTTCACTGAT |
