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. 2020 Mar 5;6(3):382–389. doi: 10.1021/acscentsci.9b00956

Figure 2.

Figure 2

MPB-sia can be metabolically engineered onto the surface of NK-92 cell to enhance the binding ability to CD22 protein. (A) Metabolic incorporation of various sialic acid derivatives onto NK-92 cells as measured by flow cytometry. Control represents nonengineered NK-92 cells treated with CD22-Fc and PE-mouse anti human CD22 mAb (Clone HIB22). (B) Quantification of the mean fluorescence intensities of cells upon incubation with various sialic acid derivatives. Mean with SD are presented for n = 3. (C) Confocal microscopy images of NK-92 cells engineered with sialic acid (sNK-92) or MPB-sia 1 (MsNK-92), followed by human CD22-Fc incubation and PE-mouse anti human CD22 mAb staining. Cells were fixed and nuclei were stained with DAPI. Scale bar, 10 μm.