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. 2020 Mar 5;6(3):382–389. doi: 10.1021/acscentsci.9b00956

Figure 4.

Figure 4

Complex formation between NK-92 cells and Raji cells investigated by confocal microscopy. Raji cells and NK-92 cells were coincubated for 1 h, fixed, permeabilized, and stained for perforin (red) to identify cytotoxic granules. Cell nuclei were labeled with DAPI (blue). Scale bar: 10 μm. (A) Representative images of cell–cell complex formation. (B) Perforin (red) fluorescence quantification. P = 0.0071. Mean with SD are presented for n = 3. Statistical significance was assessed using Student’s t test. In all figures, ns, P > 0.05; *, P < 0.05; **, P < 0.01; ***, P < 0.001.