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. 2020 Mar 20;8:181. doi: 10.3389/fcell.2020.00181

FIGURE 3.

FIGURE 3

ZetaView measurements of exosomes extracted from human ADSCs using two different isolation techniques. For the exposure experiments (A,B), exosomes were held in different concentrations of trehalose and PVP40 at 4°C temperature for 1 h. Then, the trehalose and PVP40 were removed by diafiltration using the TFF system with 1XPBS. For the freeze-drying experiments (C,D), exosomes were lyophilized in each freeze-drying solution, stored at room temperature, rehydrated with ddH2O by adding the same starting volume, and then trehalose and PVP40 were removed by diafiltration using the TFF system with 1XPBS. Finally, NTA was performed using the ZetaView instrument to measure particles diameters (A,C) and to determine the total number of particles (B,D). Data are expressed as mean ± SD (n = 6). *p < 0.0033 50 mM trehalose + 5% PVP40/LYO or 100 mM trehalose + 5% PVP40/LYO vs. 100 mM trehalose, #p < 0.0282 100 mM trehalose + 5% PVP40/LYO vs. 100 mM trehalose + 2.5%PVP40.