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Muscone represses actin-ring formation and bone resorption by osteoclasts. (A) Actin-ring formation is observed by FITC-phalloidin staining and quantified. Muscone inhibits the actin-ring formation in a dose-dependent manner. (B) Resorption area is quantified by image analysis, and muscone significantly shrinks the resorbed range in a dose-dependent manner. *P < 0.05 vs. the control group, #P < 0.05 vs. the RANKL-induced group (original scale bars, 50 μm).
