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. 2020 Mar 20;11:348. doi: 10.3389/fphar.2020.00348

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Copyright © 2020 Zhai, Yan, Zhao, Chen, Yang, Cai, He, Huang, Li, Yang, Zhou, Zhao, Wei, Bai and Li

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Muscone inhibits osteoclastogenesis only at an early stage. After induction of M-CSF (20 ng/ml) and RANKL (50 ng/ml) on day 0, BMMs and RAW264.7 cells are divided into groups according to the different times that 40 μM muscone is added. (A, C) Besides the RANKL group, BMMs are divided into three groups: day 1-7 group, day 3-7 group, and day 5-7 group, since muscone is added on day 1, 3, and 5, respectively. (B, D) And similarly, RAW264.7 cells are divided into four groups: day 0-7 group, day 1-7 group, day 2-7 group, and day 3-7 group, as muscone is added on day 0, 1, and 3, respectively. On Day 7 after RANKL stimulation, TRAP staining is performed. (E) On day 7, mRNA expression in BMMs, including MMP-9, Cathepsin K, CTR, TRAF6, TRAP, and NFATc1, are detected by RT-PCT for each group. P < 0.05 vs. the control group, #P < 0.05 vs. the RANKL-induced group.