Fig. 3.

Knock down of AK003290 reversed the effect of TSA. a, b qPCR was performed to evaluate the expression level of AK003290 and miR-124. c. d Apoptosis of cardiomyocytes was evaluated using Annexin/VI-PI staining under TSA treatment and si-AK003290 transfection. e, f MDA and LDH amount in cardiomyocytes were analysed using commercial kit. g The ROS of cardiomyocytes was detected using commercial kit and flow cytometry. h JC-1 staining was used to evaluate the MMP. i Western blot was carried out to detect the apoptotic protein expression such as bax, cytoplasma cyt-c, cleaved caspase3, and bcl-2. *p < 0.05