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. 2020 Mar 27;21:20. doi: 10.1186/s12860-020-00264-3

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — MiR-124-5p directly targets AK003290 in cardiomyocytes. a The targeting region between miR-124-5p and AK003290. b Luciferase activity assay was carried out to verify whether miR-124-5p targets AK003290 in cardiomyocytes. c qPCR was performed to detect the expression of miR-124-5p. d RNA pull down using the probe of miR-124-5p was performed to detect the interaction between miR-124-5p and AK003290. e qPCR was performed to detect the expression of AK003290. f Pearson analysis was performed to investigate the correlation between circCadm1 and Pawr as well as miR-124-5p targets AK003290. *p < 0.05