Skip to main content
. 2020 Jan 31;2(1):fcaa009. doi: 10.1093/braincomms/fcaa009

Figure 3.

Figure 3

Absence of association between presence or abundance of RNA foci and clinical manifestations or TDP-43 pathology in extra-motor regions. (A) Representative images of control (left panel) and C9orf72 (right panel) neurons of BA44 brain region associated with language and fluency dysfunction (top panel) and glial cells (bottom panel). Images demonstrate negative staining for sense RNA foci in the control tissue and discrete sense RNA foci (red dots) in neurons and glia of C9orf72 cases. Red arrows indicate cells with positive staining, n = 6 cases and six age- and sex-matched sudden death controls with no evidence of neurological disease. Scale bars = 40 µm. Residual background binding to DNA (small red dots) can be seen in controls and ALS cases; however, RNA foci are only detected in C9orf72 cases (large red RNA foci). (B) Graph quantifying number of RNA foci per cell and the proportion of cells that contain RNA foci in BA44; n = 6 cases and six age- and sex-matched sudden death controls with no evidence of neurological disease. Each bar represents a single case, error bars demonstrate variation between regions assessed within the pathological material analysed for each case. (C) Representative images of control (left panel) and C9orf72 (right panel) neurons of BA46 brain region associated with executive dysfunction (top panel) and cortical glial cells (bottom panel). Images demonstrate negative staining for sense RNA foci in the control tissue and discrete sense RNA foci (red dots) in neurons and glia of C9orf72 cases. Red arrows indicate cells with positive staining, n = 6 cases and six age- and sex-matched sudden death controls with no evidence of neurological disease. Scale bars = 40 µm. Residual background binding to DNA (small red dots) can be seen in controls and ALS cases, however RNA foci are only detected in C9orf72 cases (large red RNA foci). (D) Graph quantifying number of RNA foci per cell and the proportion of cells that contain RNA foci in BA46; n = 6 cases and six age- and sex-matched sudden death controls with no evidence of neurological disease. Each bar represents a single case, error bars demonstrate variation between regions assessed within the pathological material analysed for each case.