FIGURE 3.

Detection of infectious Leptospira spp. group and differentiation of both pathogenic groups I and II. Schematic representation of the reactions and outcome of the qPCR multiplex assessed on different templates of Leptospira spp. (A) Pathogenic group I representative strain L. interrogans denoted in red and (B) pathogenic group II L. bromii denoted in green. Primers/probe sets for each target region were denoted, 1: infectious Leptospira spp. group (also denoted in blue), 2: pathogenic group I (also denoted in red), and 3: pathogenic group II (also denoted in green). Amplification reactions specific for each target region are denoted, 1: infectious Leptospira spp. group (also denoted in blue), 2: pathogenic group I (also denoted in red), and 3: pathogenic group II (also denoted in green). In all cases, IC: internal extraction control (also denoted in yellow).