TABLE 1.
Summary of microorganism used in this study.
| No. | Specie | Serovar (strain) | Source | qPCR multiplex (infectious/path-GI/path-GII) |
| 1 | Leptospira interrogans | Autumnalis (Akiyami) | NVSL ATL-010 | Positive/positive/negative |
| 2 | Leptospira interrogans | Bratislava (Jez Bratislava | NVSL ARL-050 | Positive/positive/negative |
| 3 | Leptospira interrogans | Hardjo (Hardjoprajtino) | NVSL SJS-060 | Positive/positive/negative |
| 4 | Leptospira interrogans | Canicola (Hond Utrecht IV) | NVSL CAL-010 | Positive/positive/negative |
| 5 | Leptospira interrogans | Pomona (Pomona) | NVSL POS-010 | Positive/positive/negative |
| 6 | Leptospira interrogans (Icterohemorrhagiae) | Copenhageni (M-20) | NVSL ICL-020 | Positive/positive/negative |
| 7 | Leptospira kirschneri | Grippotyphosa (Andaman) | NVSL GRL-020 | Positive/positive/negative |
| 8 | Leptospira weilii | celledoni | ATCC® 43285TM | Positive/positive/negative |
| 9 | Leptospira broomii | – | ATCC® BAA-1107TM | Positive/negative/positive |
| 10 | Leptospira perolatiiϕ | – | IDT Integrated DNA technologies | Positive/negative/positive |
| 11 | Leptospira inadaiϕ | – | IDT Integrated DNA technologies | Positive/negative/positive |
| 12 | Leptospira biflexa | patoc 1 | ATCC® 23582TM | Negative/negative/negative |
| 13 | Salmonella enterica | Typhimurium | ATCC® 14028TM | Negative/negative/negative |
| 14 | Escherichia coli | Serotype O6, Biotype 1 | ATCC® 25922TM | Negative/negative/negative |
| 15 | Escherichia coli | – | ATCC® 35218TM | Negative/negative/negative |
| 16 | Listeria monocytogenes | – | ATCC® 7644TM | Negative/negative/negative |
| 17 | Campylobacter jejuni | – | ATCC® 33291 | Negative/negative/negative |
| 18 | Chlamydia abortus§ | – | – | Negative/negative/negative |
| 19 | Brucella abortus | – | Heat killed culture kindly provided by Illinois Department of Agriculture Animal Disease Laboratory, Galesburg, IL | negative/negative/negative |
| 20 | Brucella melitensis | Heat killed culture kindly provided by Illinois Department of Agriculture Animal Disease Laboratory, Galesburg, IL | negative/negative/negative | |
| 21 | Brucella canis | Culture stock from VDL at UIUC | negative/negative/negative | |
| 22 | Actinobacillus suis | – | Culture stock provided by NVSL check test 2008 | negative/negative/negative |
NVSL: National Veterinary Services Laboratories, United States Department of Agriculture (USDA), United States. ϕPlasmid containing the background sequence of Leptospira perolatti strain for the target regions was synthetized by IDT DNA technologies. § DNA kindly provided by Purdue University Animal Diseases Laboratory, West Lafayeette, IN, United States. VDL: Veterinary Diagnostic Laboratory. UIUC: University of Illinois at Urbana-Champaign. For a better Visualization, positive results were denoted in bold case.