Fig. 1.

Mutagenic comparison of serine-containing statistically significant pairs (SSPs) versus amino acid pairs. (a) Amino acid sequences of b12 monoclonal antibody (mAb) affinity purified phages: C10 (wild-type phage), S4 and S8 in which Ser-4 and Ser-8 are converted to alanine. The LWSDL segment of phage C10, determined to contain SSPs which are important for b12 binding, is underlined. (b) Two-fold dilutions of equal amounts of phages were dot-blotted onto a nitrocellulose membrane filter and reacted with the b12 mAb. The fth-1 phage, containing no insert, was used as a negative control, and signals were produced using enhanced chemiluminescence (from Bublil et al.,[87] with permission).