Fig. 12.

Development of viral vectors. a Design the shRNA sequences required using online algorithms and oligonucleotide companies; b clone shRNA oligonucleotides into the commercially available viral vector plasmid that suits the expression profile required; c transfect plasmids into specialised commercially available packaging cell lines that produce the replication factors required for the specific virus vector; d purify the virus that is produced, and e transduce the cells that require treatment