Table 2. Characterization of hemolysis activity of USA300, carA mutant, and complemented strains.

S. aureus strains were spotted on 5% sheeps blood TSA II and incubated at 37 °C for 24 hours followed by 24 hours at 4 °C. Plates were photographed using a lightbox and processed in ImageJ. Hemolysis rings and colonies (n = 10) for each strain were measured using ImageJ.

Strain	Average Hemolysis (mm)	Standard deviation (mm)
USA300	9.0945	.508
carA-tn	5.6450	.360
carA-tn + pOS1	5.0459	.446
carA-tn + pOS1-carA	7.3305	.536
carA-tn + pOS1-dtox null carA	7.0114	.422