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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Alcohol. 2018 Jul 26;80:17–24. doi: 10.1016/j.alcohol.2018.07.010

Fig. 1. The combination of alcohol and RSV potentiates cilia slowing coincident with PKC epsilon action in mouse tracheal epithelial cell cultures.

Fig. 1.

Ciliated mouse tracheal epithelial cells (MTEC) cultured at air-liquid interface and pretreated 24 hr with or without 100 mM alcohol (EtOH) were infected with 1 × 105 TCID50 respiratory syncytial virus (RSV) for 1–6 hr. Control cells received no treatment (Media). A) Ciliary beat frequency (CBF) decreased in RSV-treated MTEC (ap<0.05 vs Media at matched time points of 3–6 hr) while EtOH+RSV further enhanced cilia slowing due to RSV only (bp<0.05 vs RSV only at matched time points of 3–6 hr). EtOH+RSV potentiated the time of RSV-induced CBF decrease compared to media control (cp<0.01 vs Media at 1 hr). B) Protein kinase C epsilon (PKCε) activity was increased by RSV (ap<0.05 vs Media at 1 hr), enhanced by EtOH+RSV (bp<0.01 vs Media at matched time points of 1–2 hr), and significantly decreased with EtOH+RSV (cp<0.05 vs Media or RSV only at matched time points of 5–6 hr). Experiments were performed independently three times each using triplicate data measurements (n=9).