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. Author manuscript; available in PMC: 2020 Jul 15.
Published in final edited form as: Nature. 2020 Jan 15;577(7792):689–694. doi: 10.1038/s41586-019-1912-x

Extended Data Fig 8. Flow cytometry analysis of myeloid cell populations after VEGF-C treatment.

Extended Data Fig 8.

Mice bearing 7 day-tumors were treated with Luc-mRNA or VEGF-C-mRNA and evaluated for changes in myeloid populations. a Gating strategy for different myeloid cells. b-d Cell counts of different cell types were measured at different time points after VEGF-C-mRNA treatment. e-g MHCII and CD80 MFI levels were graphed and showed no significant alteration after VEGF-C-mRNA treatment. b and e are leukocytes from brain tissue. c and f are leukocytes from draining cervical lymph nodes. d and g are leukocytes from meninges (n = 3, 3 animals pooled for each replicate). Data are mean ± S.E.M *P < 0.05; **P < 0.01; ***P <0.001; ****P<0.0001 (two-tailed unpaired Student’s t-test)