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. Author manuscript; available in PMC: 2020 Sep 9.
Published in final edited form as: Nat Struct Mol Biol. 2020 Mar 9;27(3):249–259. doi: 10.1038/s41594-020-0384-x

Figure 4. Intramolecular PRE experiments for WT αSyn.

Figure 4

a) Aggregation kinetics (note the short timescale depicted) of WT αSyn (100 μM in 20 mM sodium acetate, pH 4.5, at low (20 mM added NaCl) or high (200 mM added NaCl) ionic strength at 37 °C). b-g Intramolecular PRE intensity ratios of amide protons (paramagnetic/diamagnetic) for WT αSyn variants with MTSL spin labels at A18C (b,c), A90C (d,e) or A140C (f,g) at low (b,d,f) or high (c,e,g) ionic strengths, 15 °C, as indicated. Blue, pink and red bars show intensity ratios for the N-terminal, NAC and C-terminal regions, respectively. Dark blue bars highlighted in grey point out the position of the P1/P2 region. Schematics are shown above each plot with a consistent colour scheme. The location of spin labels are denoted by a yellow circle. Grey panels highlight the location of the P1/P2 regions. Data for all graphs are available as Source Data.