Extended Data Table 1.
CryoEM data collection, refinement and validation statistics
| #1 name (EMDR-20836) (PDB 6UP7) | |
|---|---|
| Data collection and processing | |
| Magnification | 130,000 |
| Voltage (kV) | 300 |
| Electron exposure (e-/Å2) | 56 |
| Defocus range (μm) | −1.0~−2.0 |
| Pixel size (Å) | 1.06 |
| Symmetry imposed | Cl |
| Initial particle images (no.) | 12,365,655 |
| Final particle images (no.) | 254,725 |
| Map resolution (Å) | 4.2 |
| FSC threshold | 0.143 |
| Map resolution range (Å) | 3.5~5.0 |
| Refinement | |
| Initial model used (PDB code) | 4GRV,4JQI |
| Map sharpening B factor (Å2) | −164 |
| Model composition | |
| Non-hydrogen atoms | 4166 |
| Protein residues | 685 |
| Ligands | 1 |
| B factors (Å2) | |
| Protein | 69.94 |
| Ligand | 130.87 |
| R.m.s. deviations | |
| Bond lengths (Å) | 0.009 |
| Bond angles (°) | 1.362 |
| Validation | |
| MolProbity score | 2.09 |
| Clashscore | 8.44 |
| Poor rotamers (%) | 0 |
| Ramachandran plot | |
| Favored (%) | 86.81 |
| Allowed (%) | 13.19 |
| Disallowed (%) | 0 |