Table 4.
Comparison of multiplex, single primer PCR, and culture
| Detection limit at dilution step | Comparison | ||||||
|---|---|---|---|---|---|---|---|
| Single | Multi | Culture | Single/multi 35 | Single 40/single 35 | Single 40/multi 35 | Multi 35/culture | |
| AV | 10−6 | 10−6 | 10−5 | Comparable | More sensitive | More sensitive | More sensitive |
| Bp | 10−5 | 10−5 | Comparable | ||||
| Bpp | 10−3 | 10−3 | Comparable | ||||
| Cpn A4 | 10−5 | 10−4 | 10−4 | More sensitive | Comparable | More sensitive | Comparable |
| CV229E | 10−2 | 10−2 | Comparable | Comparable | Comparable | ||
| CVOC43 | 10−2 | 10−2 | Comparable | ||||
| EV (CoxB1) | 10−7 | 10−7 | 10−7 | Comparable | Comparable | Comparable | Comparable |
| IVA | 10−6 | 10−6 | 10−5 | Comparable | More sensitive | More sensitive | More sensitive |
| IVB | 10−6 | 10−6 | 10−3 | Comparable | More sensitive | More sensitive | More sensitive |
| Lpn | 10−6 | 10−6 | Comparable | ||||
| Mpn | 10−6 | 10−6 | Comparable | More sensitive | More sensitive | ||
| MPV | 10−4 | 10−4 | 10−4 | Comparable | Comparable | ||
| PIV1 | 10−4 | 10−3 | 10−6 | More sensitive | More sensitive | More sensitive | Less sensitive |
| PIV2 | 10−5 | 10−5 | Comparable | ||||
| PIV3 | 10−3 | 10−3 | 10−3 | Comparable | More sensitive | More sensitive | Comparable |
| PIV4 | 10−2 | 10−2 | Comparable | ||||
| Reo | 10−4 | 10−4 | Comparable | ||||
| RSV | 10−5 | 10−5 | 10−5 | Comparable | More sensitive | More sensitive | Comparable |
| RV | 10−4 | 10−4 | Comparable | ||||