Table 2. Cryo-EM data collection, refinement and validation statistics.
Smc3–Scc1–CtSmc1 complex (EMD-4614, PDB 6QPW) |
|
---|---|
Data collection and processing | |
Magnification | 165,000 |
Voltage (kV) | 300 |
Electron exposure (e–/Å2) | 42.08 |
Defocus range (μm) | 1.25-2.5 |
Pixel size (Å) | 0.81 |
Symmetry imposed | C1 |
Initial particle images (no.) | 290,821 |
Final particle images (no.) | 178,162 |
Map resolution (Å) | 3.2 |
FSC threshold | 0.143 |
Map resolution range (Å) | 3.2-10 |
Refinement | |
Initial model used (PDB code) | 4UX3, 6QPQ |
Model resolution (Å) | 3.2 |
FSC threshold | 0.143 |
Model resolution range (Å) | 3.2-10 |
Map sharpening B factor (Å2) | -124 |
Model composition | |
Nonhydrogen atoms | 6784 |
Protein residues | 839 |
Ligands | 4 |
B factors (Å2) | |
Protein | 85.99 |
Ligand | 53.76 |
R.m.s. deviations | |
Bond lengths (Å) | 0.005 |
Bond angles (°) | 0.987 |
Validation | |
MolProbity score | 1.66 |
Clashscore | 5.83 |
Poor rotamers (%) | 0.14 |
Ramachandran plot | |
Favored (%) | 95.02 |
Allowed (%) | 4.92 |
Disallowed (%) | 0.00 |