Table 2. Cryo-EM data collection, refinement and validation statistics.
| Smc3–Scc1–CtSmc1 complex (EMD-4614, PDB 6QPW) |
|
|---|---|
| Data collection and processing | |
| Magnification | 165,000 |
| Voltage (kV) | 300 |
| Electron exposure (e–/Å2) | 42.08 |
| Defocus range (μm) | 1.25-2.5 |
| Pixel size (Å) | 0.81 |
| Symmetry imposed | C1 |
| Initial particle images (no.) | 290,821 |
| Final particle images (no.) | 178,162 |
| Map resolution (Å) | 3.2 |
| FSC threshold | 0.143 |
| Map resolution range (Å) | 3.2-10 |
| Refinement | |
| Initial model used (PDB code) | 4UX3, 6QPQ |
| Model resolution (Å) | 3.2 |
| FSC threshold | 0.143 |
| Model resolution range (Å) | 3.2-10 |
| Map sharpening B factor (Å2) | -124 |
| Model composition | |
| Nonhydrogen atoms | 6784 |
| Protein residues | 839 |
| Ligands | 4 |
| B factors (Å2) | |
| Protein | 85.99 |
| Ligand | 53.76 |
| R.m.s. deviations | |
| Bond lengths (Å) | 0.005 |
| Bond angles (°) | 0.987 |
| Validation | |
| MolProbity score | 1.66 |
| Clashscore | 5.83 |
| Poor rotamers (%) | 0.14 |
| Ramachandran plot | |
| Favored (%) | 95.02 |
| Allowed (%) | 4.92 |
| Disallowed (%) | 0.00 |