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. Author manuscript; available in PMC: 2020 Jun 9.
Published in final edited form as: Nat Biotechnol. 2019 Dec 9;38(3):365–373. doi: 10.1038/s41587-019-0344-3

Figure 5. Smarcc2 S302A/S304A homozygous mutants show delayed neuronal differentiation.

Figure 5

a) Functional score for SMARCC2/BAF170 phosphosites. b) Design for CRISPR Knock-in mutagenesis of control (+/+), heterozygous (+/-) and homozygous (-/-) Smarcc2/Baf170 S302A/S304A mutation followed by expected neuronal differentiation timeline. c) PCA plot based on the normalized RNA-seq levels of 25,411 mouse genes obtained from of the 7 clonal lines at days 8 and 12. d) Normalized RNA-seq log counts of genes contained in signatures of Late ESC, neurons or transcriptionally regulated by REST as measured in 6 independent biological replicates presenting control (+/+), heterozygous (+/-) and homozygous (-/-) backgrounds. e) Merged immunofluorescence images of differentiated cells on day 12 stained with antibodies against neuronal microtubules Map2 (red) and Smarcc2/Baf170 (Green). Scale bar represents 25 μm.